The maker space has increasingly become an important carrier to promote the business startups and innovation strategy and an organic component to promote the national innovation.It is of rich theoretical significance to explore the evolution model of its internal corpus from the perspective of entrepreneurial ecological symbiosis. Based on the ecology and management theory, this paper first builds the Lotka-Volterra evolutionary dynamic model of the two main bodies,and reveals the current mainstream symbiosis model through numerical simulation,and builds the benchmark theory and model.Then expands the basic model to introduce the three subject symbiotic evolution model of mature enterprises to break the limitation of the mainstream two-subject symbiotic model,and discusses the impact of the presence of mature enterprises on the symbiotic subjects,subject relationships, and evolution models of the mass creation space.The research results show that,firstly, synergistic units such as start-up enterprises,supporting resource organizations and mature enterprises participate in the evolution of the whole system by reaching different symbiotic modes in the internal and external symbiotic environment of the collective creation of spatial ecosystems, and the evolution equilibrium of maker space ecosystem is influenced by the values of symbiosis coefficient between those three parts.Secondly,at present,the two-subject symbiosis model under the symbiotic environment of the maker space ecosystem has difficulty in realizing the mutual reciprocal symbiosis due to the limitation of resources and scale. The entry of mature enterprises plays the role of resource guarantee and development engine inside the system.Thirdly,the three-subject reciprocal symbiosis is the best evolution direction of it.Finally, based on the simulation results,strategic suggestions for the construction and system upgrade of domestic creative space are proposed.